Harmonic Love wave devices for biosensing applications

Simultaneous operation of a Love wave biosensor at the fundamental frequency and third harmonic, including the optimisation of IDT metallisation thickness, has been investigated. Data is presented showing a sequence of deposition and removal of a model mass layer of palmitoyl-oleoyl-sn-glycerophosphocholine (POPC) vesicles while frequency hopping between 110 and 330 MH

Reconstructed warm season temperatures for Nome, Seward Peninsula, Alaska

[1] Understanding of past climate variability in the Bering Strait region and adjacent land areas is limited by a paucity of long instrumental and paleoclimatic records. Here we describe a reconstruction of May - August temperatures for Nome, Seward Peninsula, Alaska based on maximum latewood density data which considerably extends the available climatic information. The reconstruction shows warm conditions in the late 1600s and middle-20th century and cooler conditions in the 1800s. The summer of 1783, coinciding with the Laki, Iceland volcanic event, is among the coldest in the reconstruction. Statistically significant relationships with the North Pacific Index and Bering-Chukchi sea surface temperatures indicate that the Seward tree-ring data are potentially useful as long-term indices of atmosphere-ocean variability in the region.</p

Molecular methods for the detection of food-borne pathogens an overview

Published ArticleWith various food-borne pathogens that are liable for human illnesses and in some occasions even deaths, a rapid method for detecting these pathogens has become critical, not only in the food industry for hygienic and monitoring purposes, but also to ensure the safety of consumers. Traditional methods for the detection of food-borne pathogens are cumbersome and time consuming and various rapid methods to detected food-borne pathogens have been established. Among these rapid methods described in the literature, DNA-based methods that purify pathogen DNA from food samples by phenol-chloroform extraction methods or the extraction of pathogen DNA by commercially available DNA extraction kits are commonly used. The DNA-based methods are also more sensitive and selective than the traditional methods, although many of these DNA-based methods are inhibited by food components that play a fundamental role in the sensitivity of the DNA amplification reaction. This article review methods used to extract DNA from food samples, as well as the methods used to separate and/or concentrate bacteria found in food samples

Determination of the glycoforms of human chorionic gonadotropin b-core fragment by matrix-assisted laser desoption/ionisation time of flight mass spectrometry

Background: Metabolism of human chorionic gonadotropin (hCG) in the serum and kidney yields the terminal urinary product hCG ß-core fragment (hCGßcf), comprising two disulfide-linked peptides (ß6-ß40 and ß55-ß92) of which one (ß6-ß40) retains truncated N-linked sugars. Hyperglycosylated hCGßcf may indicate choriocarcinoma or Down syndrome, but the glycosylation profile of hCGßcf has not been thoroughly evaluated. Methods: hCGßcf, purified from pregnancy urine, was reduced by "on-target" dithiothreitol (DTT) reduction and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The mass ([M+H]+) of the primary sequence of the glycosylated peptide ß6-ß40 was subtracted from the m/z values of the discrete peaks observed to give the masses of the carbohydrate moieties. Carbohydrate structure was predicted by sequentially subtracting the masses of the monosaccharide residues corresponding to N-linked carbohydrates of the hCG ß-subunit reported in the literature. Results: Mass spectra of hCGßcf revealed a broad triple peak at m/z 8700–11300. After reduction, the triple peak was replaced by a discrete set of peaks between m/z 4156 and 6354. A peak at m/z 4156.8 corresponded to the nonglycosylated peptide (ß55-ß92). The remaining nine peaks indicated that urinary hCGßcf comprises a set of glycoforms smaller and larger than the trimannosyl core. Conclusions: hCGßcf comprises a wider set of glycoforms than reported previously. Peaks of highest mass indicate evidence of hyperglycosylated carbohydrate moieties. The data support previous reports that hCGßcf oligosaccharides lack sialic acid and galactose residues. No indication was found of a ß6-ß40 peptide that was entirely devoid of carbohydrate